US 12,247,233 B2
Transaminase reactions
Gregory Hughes, Scotch Plains, NJ (US); Paul N. Devine, Tinton Falls, NJ (US); Fred J. Fleitz, Germantown, WI (US); Brendan T. Grau, Warrington, PA (US); John Limanto, Metuchen, NJ (US); Christopher Savile, Santa Clara, CA (US); and Emily Mundorff, Garden City, NY (US)
Assigned to Codexis, Inc., Redwood City, CA (US)
Filed by Codexis, Inc., Redwood City, CA (US)
Filed on May 23, 2022, as Appl. No. 17/751,200.
Application 14/547,339 is a division of application No. 13/378,963, granted, now 8,921,079, issued on Dec. 30, 2014, previously published as PCT/US2010/039343, filed on Jun. 21, 2010.
Application 17/751,200 is a continuation of application No. 16/985,080, filed on Aug. 4, 2020, granted, now 11,371,067.
Application 16/985,080 is a continuation of application No. 16/164,365, filed on Oct. 18, 2018, granted, now 10,767,202, issued on Sep. 8, 2020.
Application 16/164,365 is a continuation of application No. 15/226,397, filed on Aug. 2, 2016, granted, now 10,138,503, issued on Nov. 27, 2018.
Application 15/226,397 is a continuation of application No. 14/547,339, filed on Nov. 19, 2014, granted, now 9,434,968, issued on Sep. 6, 2016.
Claims priority of provisional application 61/219,372, filed on Jun. 22, 2009.
Claims priority of provisional application 61/308,873, filed on Feb. 26, 2010.
Prior Publication US 2022/0290197 A1, Sep. 15, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 13/00 (2006.01); C12N 9/10 (2006.01); C12P 13/04 (2006.01); C12P 17/10 (2006.01); C12P 17/12 (2006.01); C12P 17/18 (2006.01)
CPC C12P 17/10 (2013.01) [C12N 9/1096 (2013.01); C12P 13/001 (2013.01); C12P 13/04 (2013.01); C12P 17/12 (2013.01); C12P 17/182 (2013.01); C12Y 206/01 (2013.01); Y02P 20/52 (2015.11)] 8 Claims
 
1. A process for preparing an amine product of structural formula (I):

OG Complex Work Unit Chemistry
having the indicated stereochemical configuration at the stereogenic center marked with an *; in an enantiomeric excess over the opposite enantiomer, wherein
R1 is optionally substituted aryl or heteroaryl;
R2 is an optionally substituted C1-C6 alkyl, —R3C(O)R4, or —R3OC(O)R5
R3 is an optionally substituted C1-C4 alkyl, and R4 is H, an optionally substituted C1-C4 alkyl, NR6R7, or OR8, where R5, R6, R7, and R8 are independently H or C1-C4 alkyl;
the process comprising contacting a ketone substrate of structural formula (II):

OG Complex Work Unit Chemistry
with a transaminase polypeptide in the presence of an amino donor under reaction conditions suitable for converting the ketone substrate to the amine product, wherein the transaminase polypeptide comprises an amino acid sequence that has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 102 and is capable of converting the ketone substrate to the amine product of structural formula (I) at a rate that is increased as compared to SEQ ID NO:2.