	US 12,247,231 B2
	Bee-less honey
Roee Amit, Haifa (IL); Shira Levi, Haifa (IL); Lidya Tannenzapf, Haifa (IL); Yehonatan Zur, Haifa (IL); Ilan Brajzblat, Haifa (IL); Assaf Licht, Haifa (IL); Ofri Warsha, Haifa (IL); Mai Dror, Haifa (IL); Dor Ben Meir, Haifa (IL); Lior Haim, Haifa (IL); Zeinat Awwad, Haifa (IL); Nir Litver, Haifa (IL); Tzila Davidov, Haifa (IL); Liron Abrahami Pachuk, Haifa (IL); Noa Eden, Haifa (IL); Hanriet Tibi, Haifa (IL); and Orna Atar, Haifa (IL)
Assigned to TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED, Haifa (IL)
Filed by TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED, Haifa (IL)
Filed on Oct. 18, 2021, as Appl. No. 17/503,506.
Claims priority of provisional application 63/093,234, filed on Oct. 18, 2020.
Prior Publication US 2022/0117277 A1, Apr. 21, 2022
Int. Cl. C12N 1/20 (2006.01); A23L 21/25 (2016.01); C12N 9/04 (2006.01); C12N 9/24 (2006.01); C12P 19/02 (2006.01); C13K 3/00 (2006.01)

CPC C12N 9/2402 (2013.01) [A23L 21/27 (2016.08); C12N 9/0006 (2013.01); C12P 19/02 (2013.01); C13K 3/00 (2013.01); C12Y 101/0301 (2013.01); C12Y 302/01026 (2013.01)]

20 Claims

1. A kit comprising:

i. a first expression vector polynucleotide comprising at least one first promoter operatively linked to a first open reading frame, wherein said first open reading frame encodes a signal peptide and an invertase enzyme, wherein said invertase enzyme is encoded by a sequence comprising SEQ ID NO: 4 or SEQ ID NO: 5 or a sequence homologous thereto that converts one molecule of sucrose into one molecule of glucose and one molecule of fructose; and

ii. a second expression vector polynucleotide comprising a repressible promoter operatively linked to a second open reading frame, wherein said second open reading frame encodes a signal peptide and a glucose oxidase enzyme, wherein said glucose oxidase enzyme is encoded by a sequence comprising SEQ ID NO: 6 or 7 or a sequence homologous thereto that converts one glucose molecule into one molecule of glucono-1,5-lactone and one molecule of hydrogen peroxide, and wherein said repressible regulatory element inhibits transcription of said second open reading frame in response to hydrogen peroxide.