US 12,247,228 B2
Non-covalent loading of plant picovirus particles
Nicole Steinmetz, San Diego, CA (US); and Daniel Popkin, Cleveland, OH (US)
Assigned to CASE WESTERN RESERVE UNIVERSITY, Cleveland, OH (US)
Filed by CASE WESTERN RESERVE UNIVERSITY, Cleveland, OH (US)
Filed on Jun. 28, 2022, as Appl. No. 17/851,187.
Application 17/851,187 is a continuation of application No. 16/820,073, filed on Mar. 16, 2020, granted, now 11,371,025.
Application 16/820,073 is a continuation of application No. 14/769,247, granted, now 10,590,394, issued on Mar. 17, 2020, previously published as PCT/US2014/016925, filed on Feb. 18, 2014.
Claims priority of provisional application 61/857,115, filed on Jul. 22, 2013.
Claims priority of provisional application 61/767,994, filed on Feb. 22, 2013.
Prior Publication US 2023/0002742 A1, Jan. 5, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 7/00 (2006.01); A61K 31/40 (2006.01); A61K 31/473 (2006.01); A61K 33/243 (2019.01); A61K 35/76 (2015.01); A61K 47/46 (2006.01)
CPC C12N 7/00 (2013.01) [A61K 31/40 (2013.01); A61K 31/473 (2013.01); A61K 33/243 (2019.01); A61K 35/76 (2013.01); A61K 47/46 (2013.01); C12N 2770/32041 (2013.01); C12N 2770/32042 (2013.01)] 19 Claims
 
1. A nanoparticle comprising a native plant picornavirus that has been loaded with one or more cargo molecules, the one or more cargo molecules non-covalently infused into the interior of the native plant picornavirus, wherein the native plant picornavirus is loaded by contacting the native plant picornavirus in solution with a molar excess of at least about 500-fold of the cargo molecule, wherein the cargo molecule is loaded into the native plant picornavirus via simple diffusion from the solution into the picornavirus and purifying the loaded native plant picornavirus.