US 12,247,220 B2
3D printed micro-millifluidic bioreactors for long-term retinal organoid maintenance
William C. Tang, Irvine, CA (US); Andrew W. Browne, Newport Coast, CA (US); Magdalene J. Seiler, Orange, CA (US); and Yuntian Xue, Irvine, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Sep. 6, 2022, as Appl. No. 17/903,458.
Claims priority of provisional application 63/241,142, filed on Sep. 7, 2021.
Prior Publication US 2023/0077412 A1, Mar. 16, 2023
Int. Cl. C12N 5/079 (2010.01); C12M 1/00 (2006.01); C12M 1/06 (2006.01); C12M 1/34 (2006.01); C12M 3/06 (2006.01); C12N 5/0793 (2010.01)
CPC C12N 5/0621 (2013.01) [C12M 1/3476 (2013.01); C12M 23/16 (2013.01); C12M 23/22 (2013.01); C12M 27/02 (2013.01); C12M 29/10 (2013.01); C12N 5/062 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A bioreactor device comprising:
a solid substrate having a first face and a second face, the solid substrate at least partially defining a perfusion channel, a plurality of chambers, a fluidic inlet, and a fluidic outlet;
a first sheet disposed over the first face; and
a second sheet disposed over the second face, wherein the combination of the solid substrate, the first sheet, and the second sheet defining the perfusion channel and each chamber of the plurality of chambers, the plurality of chambers being arranged in rows of chambers, the perfusion channel extending from the fluidic inlet and the fluidic outlet with a serpentine path that weaves through each row of chambers such that adjacent chambers are positioned at opposite sides of the perfusion channel, each chamber being in fluid communication with the perfusion channel, wherein each chamber has an associated connecting channel that provides fluid communication between each chamber and the perfusion channel, each associated connecting channel defining a distance from the perfusion channel that eliminates fluidic movement in each chamber and reduces shear stress.