	US 12,247,218 B2
	Expandable cell populations from brain biopsies of living subjects
Matthew Olding Hebb, London (CA)
Assigned to LONDON HEALTH SCIENCES CENTRE RESEARCH INC., London (CA)
Filed by LONDON HEALTH SCIENCES CENTRE RESEARCH INC., London (CA)
Filed on Dec. 14, 2022, as Appl. No. 18/080,811.
Application 16/788,037 is a division of application No. 14/891,393, abandoned, previously published as PCT/CA2014/050461, filed on May 16, 2014.
Application 18/080,811 is a continuation of application No. 16/788,037, filed on Feb. 11, 2020, abandoned.
Claims priority of provisional application 61/824,125, filed on May 16, 2013.
Prior Publication US 2023/0113275 A1, Apr. 13, 2023
Int. Cl. C12N 5/079 (2010.01); A61K 35/30 (2015.01); G01N 33/50 (2006.01)

CPC C12N 5/0618 (2013.01) [A61K 35/30 (2013.01); G01N 33/5005 (2013.01); C12N 2510/00 (2013.01); C12N 2533/32 (2013.01)]

15 Claims

1. A method of producing a population of expanded glial progenitor brain cells characterized as expressing nestin, an oligodendrogial protein and a mesenchymal protein and that express at least one neurotrophic factor (NTF) selected from a glial cell line-derived neurotrophic factor (GDNF), a brain-derived neurotrophic factor (BDNF), or a cerebral dopamine neurotrophic factor (CDNF), the method comprising:

(a) culturing brain cells of a cortical and/or subcortical brain regions of a living subject under conditions suitable for cell expansion to obtain cultured cells,

(b) testing the cultured cells for expression of nestin, the oligodendroglial protein, the mesenchymal protein and the at least one NTF, and

(c) isolating the cultured cells that express the nestin, oligodendroglial protein, the mesenchymal protein and the at least one NTF thereby producing the population of expanded progenitor brain cells that express nestin, oligodendroglial protein, the mesenchymal protein and the at least one NTF.