	US 12,247,215 B2
	Glycosylated lysosomal proteins, method of production and uses
Paulina Dabrowska-Schlepp, Denzlingen (DE); Fode Benjamin, Denzlingen (DE); Andreas Busch, Ebringen (DE); Holger Niederkrüger, Malterdingen (DE); and Andreas Schaaf, Freiburg (DE)
Assigned to ELEVA GMBH, Freiburg (DE)
Filed by eleva GmbH, Freiburg (DE)
Filed on Apr. 25, 2022, as Appl. No. 17/728,573.
Application 17/728,573 is a division of application No. 15/542,998, granted, now 11,401,563, previously published as PCT/EP2016/055830, filed on Mar. 17, 2016.
Claims priority of application No. 15159443 (EP), filed on Mar. 17, 2015.
Prior Publication US 2022/0380791 A1, Dec. 1, 2022
Int. Cl. C12N 15/82 (2006.01); C12N 5/04 (2006.01); C12N 9/10 (2006.01); C12N 9/24 (2006.01); C12N 9/40 (2006.01)

CPC C12N 5/04 (2013.01) [C12N 9/10 (2013.01); C12N 9/2402 (2013.01); C12N 9/2465 (2013.01); C12N 15/8257 (2013.01); C12Y 302/01021 (2013.01); C12Y 302/01022 (2013.01); C12Y 302/01045 (2013.01)]

11 Claims

1. A method of manufacturing a lysosomal protein composition with paucimannosidically-modified lysosomal proteins comprising

expressing a transgene encoding a lysosomal protein in a bryophyte plant or cell, wherein the transgene of the lysosomal protein comprises a sequence encoding an N-terminal secretory signal, said lysosomal protein is expressed with an N-terminal secretory signal, and said secretory signal is optionally removed during intracellular processing, and

obtaining an expressed lysosomal protein from said bryophyte plant or cell.