US 12,247,210 B2
Methods of modulating seed and organ size in plants
Michael Bevan, Norwich (GB); and Jack Dumenil, Norwich (GB)
Assigned to Plant Bioscience Limited, Norwich (GB)
Filed by Plant Bioscience Limited, Norwich (GB)
Filed on Jul. 19, 2021, as Appl. No. 17/305,967.
Application 17/305,967 is a continuation of application No. 16/405,740, filed on May 7, 2019, granted, now 11,118,188.
Application 16/405,740 is a continuation of application No. 15/035,657, granted, now 10,337,021, issued on Jul. 2, 2019, previously published as PCT/GB2014/053296, filed on Nov. 5, 2014.
Claims priority of application No. 1319876 (GB), filed on Nov. 11, 2013.
Prior Publication US 2021/0348181 A1, Nov. 11, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A01H 5/08 (2018.01); A01H 5/00 (2018.01); A01H 5/10 (2018.01); C07K 14/415 (2006.01); C12N 15/82 (2006.01)
CPC C12N 15/8261 (2013.01) [A01H 5/00 (2013.01); A01H 5/08 (2013.01); A01H 5/10 (2013.01); C07K 14/415 (2013.01); Y02A 40/146 (2018.01)] 12 Claims
 
1. A plant, or a plant cell or a plant part thereof, with increased organ size and/or seed size, the plant or plant cell or plant part thereof comprising:
a nucleic acid sequence encoding a LIM-disrupted DA1 protein comprising an amino acid sequence having at least 90% sequence identity to one or more of SEQ ID NOs: 4 to 27 and having a disrupted or inactivated LIM domain, wherein the amino acid sequence of the LIM-disrupted DA1 protein comprises one or more sequence alterations relative to the wild-type LIM domain of SEQ ID NO: 1 that disrupt or inactivate the LIM domain, wherein the wild-type LIM domain comprises two Zn finger motifs, wherein the one or more sequence alterations abolish one or both Zn finger motifs, and wherein the plant has increased organ size and/or seed size compared to a plant without the one or more sequence alterations.