	US 12,247,208 B2
	Optimization of biomass-based fermentations
Emily Agnes Stonehouse, Etna, NH (US); John Evan Eck McBride, Lyme, NH (US); Kristen M. Deleault, Canaan, NH (US); Zachary Losordo, Norwich, VT (US); and Mitchell Codd, Lebanon, NH (US)
Assigned to DANSTAR FERMENT AG, Zug (CH)
Appl. No. 16/616,306
Filed by Lallemand Hungary Liquidity Management LLC, Budapest (HU)
PCT Filed May 23, 2018, PCT No. PCT/IB2018/053663 § 371(c)(1), (2) Date Nov. 22, 2019, PCT Pub. No. WO2018/215956, PCT Pub. Date Nov. 29, 2018.
Claims priority of provisional application 62/510,107, filed on May 23, 2017.
Prior Publication US 2020/0224209 A1, Jul. 16, 2020
Int. Cl. C12N 15/81 (2006.01); C12N 1/16 (2006.01); C12P 7/14 (2006.01)

CPC C12N 15/81 (2013.01) [C12N 1/16 (2013.01); C12P 7/14 (2013.01)]

17 Claims

1. A recombinant yeast host cell:

a) comprising a first genetic modification for expressing, in glycolytic conditions, a heterologous glycerol proton symporter STL1 protein;

b) comprising a second genetic modification for inactivating a native NAD-dependent glycerol-3-phosphate dehydrogenase (GPD) protein, wherein the native GPD protein is GPD1; and

c) comprising a third genetic modification for expressing, in high osmotic conditions, a heterologous NAD-dependent glycerol-3-phosphate dehydrogenase (GPD) protein, wherein the heterologous GPD protein is GPD2;

wherein the recombinant yeast host cell is from the genus Saccharomyces sp.; and

wherein the recombinant yeast host cell produces more ethanol and a reduced level of glycerol when compared to a wild-type host cell.