	US 12,247,198 B2
	Nucleic acid synthesis and purification device, use thereof, and nucleic acid synthesis and purification method
Xin Fang, Guangdong (CN); Xiaoluo Huang, Guangdong (CN); Yue Shen, Guangdong (CN); and Xun Xu, Guangdong (CN)
Assigned to BGI SHENZHEN, Guangdong (CN)
Appl. No. 17/311,697
Filed by BGI SHENZHEN, Guangdong (CN)
PCT Filed Dec. 14, 2018, PCT No. PCT/CN2018/121092 § 371(c)(1), (2) Date Jun. 8, 2021, PCT Pub. No. WO2020/118655, PCT Pub. Date Jun. 18, 2020.
Prior Publication US 2022/0025354 A1, Jan. 27, 2022
Int. Cl. C12N 15/10 (2006.01); B01J 19/00 (2006.01)

CPC C12N 15/10 (2013.01) [B01J 19/0046 (2013.01); B01J 2219/00497 (2013.01); B01J 2219/00759 (2013.01)]

12 Claims

1. A nucleic acid purification method, comprising:

adding a solution containing a nucleic acid to be purified into a nucleic acid purification device for incubation, the nucleic acid purification device comprising a solid support, wherein the solid support comprises a controlled pore glass (CPG), the CPG is an unmodified and bare CPG, a surface of the CPG has a hydroxyl group, and the hydroxyl group is covalently bonded directly to an oligonucleotide sequence, and wherein the nucleic acid to be purified is complementary hybridized or randomly hybridized with all of or a part of the fragment of oligonucleotide sequence in the nucleic acid purification device;

eluting the nucleic acids to be purified from the nucleic acid purification device.