US 12,247,197 B2
Systems and methods for classification of microbial cells grown in microcolonies
Samad Talebpour, Richmond Hill (CA); Anna Khimchenko, Thornhill (CA); Maryam Asadishekari, Vaughan (CA); and Stephen Wesley Leonard, Markham (CA)
Appl. No. 18/012,433
Filed by QVELLA CORPORATION, Richmond Hill (CA)
PCT Filed Jun. 25, 2021, PCT No. PCT/CA2021/050884
§ 371(c)(1), (2) Date Dec. 22, 2022,
PCT Pub. No. WO2021/258223, PCT Pub. Date Dec. 30, 2021.
Claims priority of provisional application 63/044,292, filed on Jun. 25, 2020.
Prior Publication US 2023/0235274 A1, Jul. 27, 2023
Int. Cl. G06K 9/62 (2022.01); C12N 1/20 (2006.01); G02B 21/36 (2006.01); G06T 5/50 (2006.01); G06T 5/70 (2024.01); G06V 10/147 (2022.01); G06V 10/774 (2022.01); G06V 10/82 (2022.01); G06V 20/69 (2022.01)
CPC C12N 1/20 (2013.01) [G02B 21/367 (2013.01); G06T 5/50 (2013.01); G06T 5/70 (2024.01); G06V 10/147 (2022.01); G06V 10/774 (2022.01); G06V 10/82 (2022.01); G06V 20/693 (2022.01); G06V 20/698 (2022.01); G06T 2207/10056 (2013.01); G06T 2207/20081 (2013.01); G06T 2207/20084 (2013.01); G06T 2207/20212 (2013.01); G06T 2207/30024 (2013.01)] 47 Claims
OG exemplary drawing
 
1. A method of performing classification of a microcolony, the method comprising:
employing a dark-field objective having a numerical aperture between 0.15 and 0.35 to acquire a dark-field image of the microcolony, the microcolony residing on a solid-phase growth medium; and
processing the dark-field image to classify the microcolony among to two or more microbial cell type classes based on morphological features of the microcolony.