	US 12,247,065 B2
	Method for producing a controlled mixture of two or more different antibodies
Richard Hibbert, GN Wassenaar (NL); Rob De Jong, Utrecht (NL); Aran Frank Labrijn, Nigtevecht (NL); Arnout Gerritsen, Bunnik (NL); Janine Schuurman, Diemen (NL); and Paul Parren, Odijk (NL)
Assigned to GENMAB A/S, Valby (DK)
Appl. No. 17/253,286
Filed by GENMAB A/S, Valby (DK)
PCT Filed Jun. 24, 2019, PCT No. PCT/EP2019/066594 § 371(c)(1), (2) Date Dec. 17, 2020, PCT Pub. No. WO2019/243626, PCT Pub. Date Dec. 26, 2019.
Claims priority of application No. 18179365 (EP), filed on Jun. 22, 2018.
Prior Publication US 2021/0269509 A1, Sep. 2, 2021
Int. Cl. C07K 16/00 (2006.01); C07K 1/18 (2006.01); C07K 1/22 (2006.01)

CPC C07K 16/00 (2013.01) [C07K 1/18 (2013.01); C07K 1/22 (2013.01); C07K 2317/21 (2013.01); C07K 2317/24 (2013.01); C07K 2317/31 (2013.01)]

36 Claims

1. A method for producing an output mixture of two or more different antibodies having a difference in their amino acid sequences, which difference enables separation of the antibodies by chromatography, wherein the difference in the amino acid sequence of said two or more different antibodies results in a difference in (i) the charge properties of the two or more antibodies so that the two or more antibodies interact differently with a chromatography resin, (ii) the hydrophobic properties of the two or more antibodies so that the two or more antibodies interact differently with a chromatography resin, or (iii) affinity for a chromatography resin, and wherein

the two or more different antibodies are present in said output mixture at, or essentially at, a desired or predetermined concentration ratio; and

the method comprises the steps of:

(a) providing an input mixture wherein the two or more different antibodies are not present at, or essentially at, the desired or predetermined concentration ratio;

(b) separating the two or more antibodies by said chromatography; and