US 12,247,049 B1
Apparatus and methods for solid phase peptide synthesis to minimize solvent utilization
Yoheng Hanson Chang, Foster City, CA (US); and Dario Slavazza, Fremont, CA (US)
Assigned to CSBIO INSTRUMENTATION CO., Mountain View, CA (US)
Filed by CSBio Instrumentation Co., Mountain View, CA (US)
Filed on Apr. 23, 2024, as Appl. No. 18/643,247.
Int. Cl. C07K 1/04 (2006.01); B01J 19/00 (2006.01); C07K 1/06 (2006.01)
CPC C07K 1/045 (2013.01) [B01J 19/0046 (2013.01); C07K 1/061 (2013.01); B01J 2219/00495 (2013.01); B01J 2219/00596 (2013.01); B01J 2219/00725 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A method for reusing wash solvent used in washing deprotecting agent from a reaction vessel containing a solid support in a chemical synthesis device, the method comprising:
delivering wash solvent to the reaction vessel to mix with the solid support therein;
removing the used wash solvent from the reaction vessel, the used wash solvent including deprotecting agent and the wash solvent;
transferring the used wash solvent to an evaporator;
evaporating away the deprotecting agent from the evaporator, leaving purified wash solvent;
transferring the purified wash solvent directly from the evaporator back to the reaction vessel as the wash solvent for a subsequent mixing with the solid support in the reaction vessel; and
repeating the removing, the transferring the used wash solvent, the evaporating and the transferring the purified wash solvent steps until a desired level of removal of the deprotecting agent in the solid support in the reaction vessel is obtained.