US 12,247,009 B2
Crosslinkers for mass spectrometric identification and quantitation of interacting peptides
Jie Luo, Seattle, WA (US); and Jeff Ranish, Seattle, WA (US)
Assigned to Institute for Systems Biology, Seattle, WA (US)
Appl. No. 16/083,397
Filed by Institute for Systems Biology, Seattle, WA (US)
PCT Filed Mar. 10, 2017, PCT No. PCT/US2017/021946
§ 371(c)(1), (2) Date Sep. 7, 2018,
PCT Pub. No. WO2017/156480, PCT Pub. Date Sep. 14, 2017.
Claims priority of provisional application 62/306,555, filed on Mar. 10, 2016.
Prior Publication US 2019/0084929 A1, Mar. 21, 2019
Int. Cl. C07D 207/416 (2006.01); C07D 207/46 (2006.01); C07D 513/04 (2006.01); G01N 33/60 (2006.01); G01N 33/68 (2006.01)
CPC C07D 207/416 (2013.01) [C07D 207/46 (2013.01); C07D 513/04 (2013.01); G01N 33/60 (2013.01); G01N 33/6845 (2013.01); G01N 33/6848 (2013.01); G01N 33/6851 (2013.01); G01N 2458/15 (2013.01); G01N 2560/00 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A crosslinker for identifying specifically interacting proteins and the sites of crosslinking by mass spectrometry (MS) which crosslinker has the formula (1):
PBG-X1—Y—Z—Y—X2-PBG  (1)
wherein each protein binding group (PBG) reacts with an amino, sulfhydryl, hydroxyl or carboxyl group;
X1 and X2 are structurally identical linkers containing 3-15 linking member atoms selected from C, N, O and S, and wherein X1 and X2 contain different isotopes such that the mass of X1 differs from the mass of X2;
both Y are bonds cleavable under the same MS conditions as peptide bonds; and
Z is a linker containing 1-20 linking members selected from C, N, O and S.