US 12,246,321 B2
Nanosensor methods and apparatuses for determination of analytes
Qimin Quan, Cambridge, MA (US); and Feng Liang, Cambridge, MA (US)
Assigned to President and Fellows of Harvard College, Cambridge, MA (US)
Appl. No. 16/644,148
Filed by President and Fellows of Harvard College, Cambridge, MA (US)
PCT Filed Sep. 7, 2018, PCT No. PCT/US2018/049883
§ 371(c)(1), (2) Date Mar. 3, 2020,
PCT Pub. No. WO2019/051181, PCT Pub. Date Mar. 14, 2019.
Claims priority of provisional application 62/556,186, filed on Sep. 8, 2017.
Prior Publication US 2021/0001330 A1, Jan. 7, 2021
Int. Cl. B01L 3/00 (2006.01); G01N 21/25 (2006.01); G01N 21/552 (2014.01)
CPC B01L 3/5085 (2013.01) [G01N 21/25 (2013.01); G01N 21/554 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/141 (2013.01); B01L 2300/044 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0893 (2013.01); B01L 2300/0896 (2013.01); B01L 2300/123 (2013.01); G01N 2201/06113 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A method, comprising:
allowing a lysing reagent to enter a well of a microwell array through a semipermeable membrane sealing the well, wherein the semipermeable membrane is configured to prevent a lysate from leaving the well;
lysing a cell within the well to release an analyte suspected of being able to bind a reaction entity;
applying electromagnetic radiation to a nanoparticle at least partially coated with the reaction entity, wherein: (i) the nanoparticle is configured to interact with the electromagnetic radiation via electric resonance and/or magnetic resonance to alter the electromagnetic radiation; and (ii) the well comprises a nanostructure and the nanoparticle is positioned distally on an end of the nanostructure; and
determining the altered electromagnetic radiation.