US 12,246,305 B2
Systems and methods for chromatography use and regeneration
Robert Stairs, High Springs, FL (US); James Reilly, Putnam Valley, NY (US); John Mattila, Nyack, NY (US); and Samantha Wadsworth, Stamford, CT (US)
Assigned to Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Appl. No. 17/753,915
Filed by Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
PCT Filed Sep. 23, 2020, PCT No. PCT/US2020/052243
§ 371(c)(1), (2) Date Mar. 18, 2022,
PCT Pub. No. WO2021/061790, PCT Pub. Date Apr. 1, 2021.
Claims priority of provisional application 62/958,899, filed on Jan. 9, 2020.
Claims priority of provisional application 62/905,033, filed on Sep. 24, 2019.
Prior Publication US 2022/0323937 A1, Oct. 13, 2022
Int. Cl. B01D 15/20 (2006.01); B01D 15/32 (2006.01); B01J 20/34 (2006.01); C07K 1/20 (2006.01); C07K 16/06 (2006.01)
CPC B01J 20/3475 (2013.01) [B01D 15/203 (2013.01); B01D 15/327 (2013.01); C07K 1/20 (2013.01); C07K 16/065 (2013.01); B01J 2220/58 (2013.01)] 24 Claims
OG exemplary drawing
 
1. A method of regenerating a hydrophobic interaction chromatography column to which a load mass has been applied, the method comprising:
passing one or more column volumes of an alkaline solution through hydrophobic interaction media within the column, wherein the alkaline solution exhibits a pH of between about 10 and about 14, and a conductivity of between about 0.5 mS/cm and about 10 mS/cm,
wherein material bound to the hydrophobic interaction media is removed.