	US 12,246,110 B2
	In vitro method for creating a viable connective tissue and/or osseous tissue
Michel Hassler, Saint Ismier (FR); and Ghassene Ouenzerfi, Saint Martin d'Hères (FR)
Assigned to TORNIER, Montbonnot Saint Martin (FR)
Filed by TORNIER, Montbonnot Saint Martin (FR)
Filed on Jan. 10, 2023, as Appl. No. 18/152,470.
Application 18/152,470 is a continuation of application No. 16/772,712, granted, now 11,577,000, previously published as PCT/EP2018/085148, filed on Dec. 17, 2018.
Claims priority of application No. 17306796 (EP), filed on Dec. 18, 2017.
Prior Publication US 2023/0158207 A1, May 25, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A61L 27/36 (2006.01); C12M 1/00 (2006.01); C12M 1/42 (2006.01); C12M 3/00 (2006.01); C12N 5/077 (2010.01)

CPC A61L 27/3612 (2013.01) [A61L 27/3654 (2013.01); A61L 27/3691 (2013.01); C12M 21/08 (2013.01); C12M 23/20 (2013.01); C12M 35/04 (2013.01); C12N 5/0654 (2013.01); C12N 5/0655 (2013.01); C12N 2527/00 (2013.01)]

11 Claims

1. An in vitro method for creating a viable connective tissue and/or osseous tissue obtained by tribological solicitations of a biological culture to repair a patient's bone defect, the method comprising:

(a) culturing a blood sample or biopsy from the patient on a pyrocarbon (Pyc) applicator in the presence of a tissue culture media in a bioreactor for at least about 4 days thus resulting in tissue that is growing, wherein the Pyc applicator has a matched convex/concave shape that mimics a shape of the patient's bone defect to be repaired, and concomitantly, afterwards, or with an overlap of, wherein the blood sample or biopsy of the patient includes stem cells;

(b0) submitting the growing tissue obtained at step (a) to a perpendicular pressure force for at least 4 days to obtain an osseous tissue, and concomitantly, after or with an overlap;

(b1) submitting the growing tissue obtained at step (a) and the osseous tissue to a perpendicular pressure force and a movement parallel to a surface of the pyrocarbon applicator for at least 4 days to obtain a connective tissue; and