| CPC A61K 31/519 (2013.01) [A61K 9/1271 (2013.01); A61K 9/1277 (2013.01); A61K 31/282 (2013.01); A61K 39/3955 (2013.01); A61K 47/26 (2013.01); A61K 47/645 (2017.08); A61K 47/6913 (2017.08); A61P 35/00 (2018.01)] | 26 Claims |

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1. A liposomal composition comprising a gamma polyglutamated pemetrexed encapsulated by a liposome, wherein the gamma polyglutamated pemetrexed has 4, 5, 2-10, 4-6, or more than 5, glutamyl groups having a gamma carboxyl group linkage, and wherein the liposome is pegylated, wherein the liposome does not contain a targeting moiety having specific affinity for a surface antigen on a target cell,
wherein the liposome does not contain a cell penetrating peptide and does not contain a mitochondria penetrating peptide,
wherein the liposome has a zeta potential that is less than or equal to zero, between 0 to −150 mV, or between −30 to −50 mV, and
wherein the liposome is capable of delivering the gamma polyglutamated pemetrexed directly into a cell.
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