US 12,245,866 B2
Label-free spectral pathology for in vivo diagnosis
James Chan, Davis, CA (US); and Payam Saadai, Davis, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Appl. No. 17/775,231
Filed by The Regents of the University of California, Oakland, CA (US)
PCT Filed Nov. 6, 2020, PCT No. PCT/US2020/059539
§ 371(c)(1), (2) Date May 6, 2022,
PCT Pub. No. WO2021/092489, PCT Pub. Date May 14, 2021.
Claims priority of provisional application 62/931,905, filed on Nov. 7, 2019.
Prior Publication US 2022/0386939 A1, Dec. 8, 2022
Int. Cl. A61B 5/00 (2006.01); A61B 1/31 (2006.01); G01J 3/44 (2006.01); G01N 21/65 (2006.01)
CPC A61B 5/4255 (2013.01) [A61B 1/31 (2013.01); A61B 5/0071 (2013.01); A61B 5/0075 (2013.01); A61B 5/4029 (2013.01); A61B 5/4893 (2013.01); A61B 5/725 (2013.01); G01J 3/44 (2013.01); G01N 21/65 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method for determining if a tissue is ganglionic or non-ganglionic comprising:
a) identifying a region of interest in a tissue suspected to contain ganglion cells by at least one of autofluorescence (AF) or Second Harmonic generation (SHG) imaging of the tissue;
b) generating at least one hyperspectral Raman image of a tissue from the region of interest; and
c) identifying the tissue as ganglionic or non-ganglionic by comparing a Raman signature intensity from the hyperspectral Raman image of the region of interest to a respective Raman signature of an adjacent tissue, wherein the Raman signature comprises at least one of 710 cm−1±1 cm−1, 734 cm−1±1 cm−1, 797 cm−1±1 cm−1, 859 cm−1±1 cm−1, 924 cm−1±1 cm−1 1216 cm−1±1 cm−1, 1362 cm−1±1 cm−1, 1440 cm−1±1 cm−1, or 1660 cm−1=1 cm−1 and an intensity peak determines ganglionic tissue from a non-ganglionic tissue.
 
14. A method of detecting ganglion cells from a tissue of a subject, comprising:
evaluating the tissue using at least one of second harmonic generation or autofluorescence to detect at least one candidate region potentially having ganglion cells; and
evaluating the at least one candidate region relative to a region of the tissue adjacent to the at least one candidate region using Raman spectroscopy to determine whether the at least one candidate region has a ganglion cell;
wherein the evaluating using autofluorescence comprises detecting the at least one candidate region as a region of lower autofluorescence relative to the tissue adjacent to the at least one candidate region.