US 12,245,855 B2
Aptamer based portable diagnostic medical device and methods of use
Ismael Marvin Voltaire, Hollywood, FL (US)
Assigned to Prodia Technologies, Inc., Haines City, FL (US)
Filed by Ismael Marvin Voltaire, Hollywood, FL (US)
Filed on Dec. 5, 2023, as Appl. No. 18/529,227.
Application 18/529,227 is a division of application No. 16/699,534, filed on Nov. 29, 2019, granted, now 11,896,369.
Claims priority of provisional application 62/834,957, filed on Apr. 16, 2019.
Claims priority of provisional application 62/772,389, filed on Nov. 28, 2018.
Claims priority of application No. 119400405 (SA), filed on Jan. 28, 2019.
Prior Publication US 2024/0099610 A1, Mar. 28, 2024
Int. Cl. A61B 5/145 (2006.01); A61B 5/00 (2006.01); A61B 5/1455 (2006.01)
CPC A61B 5/14514 (2013.01) [A61B 5/0024 (2013.01); A61B 5/14503 (2013.01); A61B 5/14546 (2013.01); A61B 5/14556 (2013.01); A61B 5/6824 (2013.01); A61B 5/6828 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A method for detecting and measuring one or more biomarker in a biological sample from a subject, the method comprising:
a) attaching a measurement device to the subject, the measurement device having a sample access component containing microneedles, a sample pre-processing filter, a sample pre-processing component, a sensor component, and an electronic component;
b) engaging the microneedles of the sample access component with the skin of the subject, such that the microneedles penetrate the epidermis layer of the skin;
c) selectively removing the biological sample from the subject via the microneedles;
d) drawing the biological sample through a microchannel of the measurement device in a direction toward the sensor component;
e) conveying the biological sample through a pre-processing filter wherein the filter has a pore size that is no larger than the largest biomarker being assessed;
f) further conveying the biological sample through a pre-processing component of the measuring device, the pre-processing component having at least one first aptamer-based magnetic bead conjugate (“aptamer-MB”) that is configured to chemically bind to at least one non-desired biomarker, the at least one non-desired biomarker being different from the one or more biomarker;
g) removing at least one non-desired biomarker from the biological sample by the at least one first aptamer-MB of the pre-processing component of the measurement device;
h) passing the biological sample through the sensor component of the measurement device, the sensor component containing a plurality of quartz crystal plates, each of which is outfitted with at least one aptamer configured to chemically bind to the one or more biomarker;
i) analyzing the at least one aptamer by a microprocessor of the electronic component of the measurement device, the microprocessor being configured to determine the presence and amount of the one or more biomarker; and
j) transmitting data associated with the presence and amount of the one or more biomarker by the microprocessor of the measurement device.