US 12,245,793 B2
Robotic microtool control in an intelligent automated in vitro fertilization and intracytoplasmic sperm injection platform
Gerardo Mendizabal-Ruiz, Guadalajara (MX); Joshua Abram, Lyme, CT (US); Adolfo Flores-Saiffe Farias, Zapopan (MX); Cesar Millan, Zapopan (MX); Roberto Valencia-Murillo, Las Paz (MX); Vladimir C. Ocegueda Hernandez, Zapopan (MX); Estefania Hernandez, Guadalajara (MX); Victor Manuel Medina Perez, Guadalajara (MX); Alejandro Chavez-Badiola, Mexico City (MX); Alan Murray, Greenwich, CT (US); and Jacques Cohen, New York, NY (US)
Assigned to Conceivable Life Sciences Inc., New York, NY (US)
Filed by Conceivable Life Sciences Inc., New York, NY (US)
Filed on Feb. 2, 2024, as Appl. No. 18/430,762.
Application 18/430,762 is a continuation of application No. PCT/US2024/013428, filed on Jan. 30, 2024.
Claims priority of provisional application 63/523,258, filed on Jun. 26, 2023.
Prior Publication US 2024/0426856 A1, Dec. 26, 2024
Int. Cl. G01N 35/00 (2006.01); A61B 17/425 (2006.01); A61B 17/43 (2006.01); A61B 34/30 (2016.01); C12M 1/00 (2006.01); C12M 1/02 (2006.01); C12M 1/22 (2006.01); C12M 1/26 (2006.01); C12M 1/36 (2006.01); C12M 3/00 (2006.01); C12N 5/073 (2010.01); G01N 33/50 (2006.01); G01N 35/10 (2006.01); G06T 7/00 (2017.01); G06T 7/20 (2017.01); G06T 7/70 (2017.01); G06V 10/82 (2022.01); G06V 20/69 (2022.01); A61B 34/32 (2016.01)
CPC A61B 17/43 (2013.01) [A61B 17/425 (2013.01); A61B 34/30 (2016.02); C12M 21/06 (2013.01); C12M 23/10 (2013.01); C12M 23/48 (2013.01); C12M 23/50 (2013.01); C12M 33/04 (2013.01); C12M 41/06 (2013.01); C12M 41/18 (2013.01); C12M 41/48 (2013.01); C12N 5/0604 (2013.01); G01N 33/5091 (2013.01); G01N 35/0099 (2013.01); G01N 35/10 (2013.01); G01N 35/1011 (2013.01); G06T 7/20 (2013.01); G06T 7/70 (2017.01); G06V 10/82 (2022.01); G06V 20/693 (2022.01); A61B 34/32 (2016.02); G06T 2207/20081 (2013.01); G06T 2207/30024 (2013.01); G06V 20/69 (2022.01); G06V 20/698 (2022.01); G06V 2201/03 (2022.01)] 6 Claims
OG exemplary drawing
 
1. A method of artificial intelligence-based robotic pipetting for spermatozoa preparation, comprising:
positioning a vessel containing a semen sample;
receiving by an artificial intelligence/machine learning system (AI/ML system) an image object from an imaging system that includes a microscopy system, a camera system, and a lighting system;
determining a semen liquefaction state of the semen sample based at least in part on the image object, by comparing the determined semen liquefaction state to a stored liquefaction state criterion using the AI/ML system to confirm the adequacy of the determined liquefaction state for spermatozoa collection;
determining, using the AI/ML system, a sperm characteristic within the semen sample based at least in part on the image object;
comparing the determined sperm characteristic and motility fraction to a stored sperm criterion using the AI/ML system to confirm the adequacy of the determined sperm characteristic and motility fraction for spermatozoa collection;
determining, using the AI/ML system, a rate of spermatozoa movement based at least in part on the image object;
comparing the determined rate of spermatozoa movement to a stored movement criterion using the AI/ML system to confirm the adequacy of the determined rate of spermatozoa movement for spermatozoa separation;
using the AI/ML system to instruct a robotic pipettor to collect a sperm within the semen sample upon confirmation of adequacy of the determined liquefaction state, determined sperm characteristic and motility fraction, and determined spermatozoa movement; and
collecting at least one sperm within the robotic pipettor.