US 12,245,740 B2
Systems and methods to repair tissue defects
Darryl D. D'Lima, San Diego, CA (US); and Clifford W. Colwell, Jr., La Jolla, CA (US)
Assigned to SCRIPPS HEALTH, San Diego, CA (US)
Filed by SCRIPPS HEALTH, San Diego, CA (US)
Filed on Oct. 10, 2022, as Appl. No. 18/045,377.
Application 18/045,377 is a continuation of application No. 16/304,618, granted, now 11,497,831, previously published as PCT/US2017/034539, filed on May 25, 2017.
Claims priority of provisional application 62/341,914, filed on May 26, 2016.
Prior Publication US 2023/0233058 A1, Jul. 27, 2023
Int. Cl. A61L 27/20 (2006.01); A61B 1/00 (2006.01); A61B 34/30 (2016.01); A61B 90/00 (2016.01); A61F 2/08 (2006.01); A61F 2/30 (2006.01); A61L 27/02 (2006.01); A61L 27/24 (2006.01); A61L 27/36 (2006.01); A61M 5/142 (2006.01); A61M 5/20 (2006.01); B29C 64/112 (2017.01); B33Y 10/00 (2015.01); B33Y 70/00 (2020.01); C12N 5/077 (2010.01); A61B 17/00 (2006.01)
CPC A61B 1/00009 (2013.01) [A61B 1/00 (2013.01); A61B 34/30 (2016.02); A61B 90/00 (2016.02); A61F 2/0805 (2013.01); A61F 2/0811 (2013.01); A61F 2/30756 (2013.01); A61F 2/3094 (2013.01); A61L 27/025 (2013.01); A61L 27/20 (2013.01); A61L 27/24 (2013.01); A61L 27/3604 (2013.01); A61L 27/3608 (2013.01); A61L 27/3612 (2013.01); A61M 5/14212 (2013.01); A61M 5/20 (2013.01); B29C 64/112 (2017.08); B33Y 10/00 (2014.12); B33Y 70/00 (2014.12); C12N 5/0654 (2013.01); C12N 5/0655 (2013.01); A61B 2017/00969 (2013.01); A61B 2090/395 (2016.02); A61F 2002/30962 (2013.01); A61L 2430/06 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of bioprinting a bio-ink construct on an internal tissue defect during a minimally invasive surgery on an individual in need thereof, comprising:
a. visualizing the internal tissue defect;
b. positioning a bioprinter comprising: a printhead, a bio-ink reservoir, and a nozzle within proximity of or in contact with the internal tissue defect, wherein the bio-ink reservoir is fluidically connected to the nozzle, and wherein the bio-ink reservoir contains a bio-ink comprising a plurality of cells and a photoinitiator; and
c. ejecting the bio-ink from the printhead onto the internal tissue defect to form a bio-ink layer, thereby generating a bio-ink construct.