US 12,245,576 B2
Method for genetic manipulation of sap-feeding insects
Peter W. Atkinson, Riverside, CA (US); and Linda L. Walling, Riverside, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Appl. No. 16/960,285
Filed by The Regents of the University of California, Oakland, CA (US)
PCT Filed Jan. 9, 2019, PCT No. PCT/US2019/012939
§ 371(c)(1), (2) Date Jul. 6, 2020,
PCT Pub. No. WO2019/140010, PCT Pub. Date Jul. 18, 2019.
Claims priority of provisional application 62/734,953, filed on Sep. 21, 2018.
Claims priority of provisional application 62/615,826, filed on Jan. 10, 2018.
Prior Publication US 2021/0105986 A1, Apr. 15, 2021
Int. Cl. A01K 67/033 (2006.01); G01N 33/50 (2006.01)
CPC A01K 67/0339 (2013.01) [G01N 33/5085 (2013.01); A01K 2217/07 (2013.01); A01K 2227/706 (2013.01); A01K 2267/03 (2013.01)] 5 Claims
 
1. An assay system for testing the effect of a chemical on an insect comprising:
a rooted leaf-disc;
an agar; and
a solution comprising Gamborg's (GB) media supplemented with Gamborg's vitamins (GBV).