	US 12,245,562 B2
	System for rapid, robust, and efficient in vitro mass propagation of Miscanthus x giganteus
Bertrand B. Hankoua, Dover, DE (US); and Ayalew Ligaba Osena, Greensboro, NC (US)
Assigned to Delaware State University, Dover, DE (US)
Filed by Delaware State University, Dover, DE (US)
Filed on Feb. 21, 2023, as Appl. No. 18/112,271.
Application 18/112,271 is a division of application No. 17/482,723, filed on Sep. 23, 2021, granted, now 11,589,526.
Application 17/482,723 is a division of application No. 16/745,883, filed on Jan. 17, 2020, granted, now 11,154,023.
Application 16/745,883 is a continuation of application No. PCT/US2018/043130, filed on Jul. 20, 2018.
Claims priority of provisional application 62/534,935, filed on Jul. 20, 2017.
Prior Publication US 2023/0200323 A1, Jun. 29, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A01H 4/00 (2006.01); A01G 20/00 (2018.01); A01H 5/12 (2018.01); A01H 6/46 (2018.01)

CPC A01H 4/005 (2013.01) [A01G 20/00 (2018.02); A01H 5/12 (2013.01); A01H 6/46 (2018.05)]

4 Claims

1. Multiple in vitro tillers of Miscanthus x giganteus (Giant miscanthus) produced from a cluster of stem segments containing shoot primordia (CSSSP) according to a method,

wherein the CSSSP comprises a plurality of stem segments and is generated by partial removal of in vitro tillers from a cluster of multiple in vitro shoots (CMIT), and each of the plurality of stem segments is a basal portion of one of the removed in vitro tillers and comprises a shoot primordium, and

wherein the method comprises fragmenting the CSSSP into small clusters of stem segments, wherein the small clusters of stem segments comprise a plurality of shoot primordia, and incubating each of the small clusters in a direct shoot induction medium (DSIM), and developing multiple in vitro tillers of Miscanthus x giganteus (Giant miscanthus) from the plurality of shoot primordia in each of the small clusters of stem segments.