US 11,921,275 B2
Illumination filter system and observation system for a multispectral fluorescence microscope, multispectral fluorescence microscope, and microscopying method
Manfred Kuster, Widnau (CH); and George Themelis, Lindau (DE)
Assigned to Leica Instruments (Singapore) Pte. Ltd., Singapore (SG)
Filed by LEICA INSTRUMENTS (SINGAPORE) PTE. LTD., Singapore (SG)
Filed on Oct. 1, 2020, as Appl. No. 17/060,665.
Application 17/060,665 is a continuation of application No. 15/424,815, filed on Feb. 4, 2017, granted, now 10,823,947.
Claims priority of application No. 16155623 (EP), filed on Feb. 15, 2016.
Prior Publication US 2021/0018738 A1, Jan. 21, 2021
Int. Cl. G02B 21/16 (2006.01); A61B 1/00 (2006.01); A61B 1/04 (2006.01); A61B 1/045 (2006.01); A61B 1/06 (2006.01); A61B 5/00 (2006.01); G01N 21/64 (2006.01); G02B 5/28 (2006.01); G02B 21/00 (2006.01)
CPC G02B 21/16 (2013.01) [A61B 1/00186 (2013.01); A61B 1/043 (2013.01); A61B 1/045 (2013.01); A61B 1/0646 (2013.01); A61B 5/0071 (2013.01); G01N 21/6458 (2013.01); G02B 5/28 (2013.01); G02B 21/0012 (2013.01); G01N 2021/6419 (2013.01); G01N 2021/6471 (2013.01); G01N 2201/061 (2013.01)] 15 Claims
OG exemplary drawing
 
1. An observation system for multispectral fluorescence medical imaging, the observation system comprising:
a beam splitter arranged to receive a light image from an illuminated object, wherein the beam splitter is adapted to split the light image into a first light portion along a first light path and a second light portion along a second light path;
a first sensor in the first light path for capturing the first light portion;
a second sensor in the second light path for capturing the second light portion;
wherein the light image comprises fluorescence light emitted from the object and visible reflected light reflected from the object;
wherein the fluorescence light of the light image includes a first fluorescence emission band and a second fluorescence emission band separated from the first fluorescence emission band by an intermediate band;
wherein the first fluorescence emission band is in the visible spectrum;
wherein the first light portion comprises the fluorescence light of the light image, and the second light portion comprises the visible reflected light of the light image and not the fluorescence light of the light image; and
wherein the beam splitter is adapted to include at least a portion of the intermediate band in the second light portion.