US 11,921,108 B2
Methods for determining amount of functional albumin
Sebastian Klammt, Berlin (DE); Steffen Mitzner, Rostock (DE); Jan Stange, Rostock (DE); and Emil Reisinger, Rostock (DE)
Assigned to FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V., Munich (DE)
Filed by Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V., Munich (DE)
Filed on Feb. 8, 2022, as Appl. No. 17/667,376.
Application 17/667,376 is a division of application No. 16/607,064, granted, now 11,280,780, previously published as PCT/EP2018/060176, filed on Apr. 20, 2018.
Claims priority of application No. 10 2017 206 786.1 (DE), filed on Apr. 21, 2017.
Prior Publication US 2022/0163517 A1, May 26, 2022
Int. Cl. G01N 33/53 (2006.01); G01N 33/543 (2006.01); G01N 33/68 (2006.01)
CPC G01N 33/54306 (2013.01) [G01N 33/6803 (2013.01); G01N 2333/765 (2013.01)] 13 Claims
 
1. A method for determining an amount of functional albumin, the method comprising:
a) providing a test sample containing a defined amount of albumin of unknown binding capacity and a reference sample containing the same defined amount of albumin having a reference binding capacity;
b) incubating the test sample and reference sample with a defined amount of at least one albumin-binding marker M under conditions that allow the at least one albumin-binding marker M to bind to albumin (A) to form complexes of the at least one albumin-binding marker M and albumin (M:A);
c) removing the complexes (M:A) formed in step c);
d) detecting a presence or an amount of unbound marker M in the samples after removal of the complexes (M:A) through first and second test strips that allow for a determination of an amount of unbound marker M, wherein the first and the second test strips have different detection limits/tolerance limits for the marker M, wherein said detecting is qualitative or semiquantitative detecting in which the unbound marker is detected based on the detection limit of the test strip; and
e) determining the amount of functional albumin based on the presence or the amounts detected of marker M in each of the first and second test strips in step d).