US 11,921,041 B2
Method and system for visualization of endotoxins in a fluid sample
Sukmi Ledru, Boulder, CO (US); Krzysztof Franaszczuk, Boulder, CO (US); Jesse Manley, Boulder, CO (US); David Kremer, Boulder, CO (US); Vamsi Panuganti, Boulder, CO (US); Jeremiah Teague, Boulder, CO (US); Yan Le, Boulder, CO (US); and Michael Scaer, Boulder, CO (US)
Assigned to BL Technologies, Inc., Minnetonka, MN (US)
Appl. No. 17/437,117
Filed by BL TECHNOLOGIES, iNC., Minnetonka, MN (US)
PCT Filed Mar. 9, 2020, PCT No. PCT/US2020/021642
§ 371(c)(1), (2) Date Sep. 8, 2021,
PCT Pub. No. WO2020/185646, PCT Pub. Date Sep. 17, 2020.
Claims priority of provisional application 62/826,355, filed on Mar. 29, 2019.
Claims priority of provisional application 62/815,716, filed on Mar. 8, 2019.
Prior Publication US 2022/0178826 A1, Jun. 9, 2022
Int. Cl. G01N 21/64 (2006.01); G01N 21/27 (2006.01); G01N 21/77 (2006.01); G01N 33/579 (2006.01); G16H 10/40 (2018.01); G16H 15/00 (2018.01); G16H 20/17 (2018.01); G16H 40/63 (2018.01)
CPC G01N 21/6452 (2013.01) [G01N 21/272 (2013.01); G01N 21/77 (2013.01); G01N 33/579 (2013.01); G16H 10/40 (2018.01); G16H 15/00 (2018.01); G16H 20/17 (2018.01); G16H 40/63 (2018.01); G01N 2201/122 (2013.01)] 25 Claims
OG exemplary drawing
 
1. A computer-implemented method to generate visualizations of endotoxin concentrations in a fluid sample, the method comprising:
generating, by a processor, for a graphical user interface or for a report, to be displayed on a stationary or mobile computing device, a graphical visualization, from a data set that identifies an optical density (OD) reading of a microplate comprising a plurality of optical wells, each optical well containing a fluid sample, said graphical visualization comprising a vertically-oriented bar graph having a plurality of vertical bars with at least one vertically-oriented bar graph for each optical well of the microplate,
wherein the processor causes the vertically-oriented bar graph corresponding to the optical well of the microplate to increase vertically over time as a reaction between Limulus Amoebocyte Lysate (LAL) and bacterial endotoxin takes place in the optical well of the microplate,
wherein a greater amount of endotoxin present in the optical well, the faster the reaction occurs with the LAL reagent, and subsequently the faster the processor causes the associated vertically-oriented bar graph to increase on the graphical visualization.