US 11,920,184 B2
Concentrating a target molecule for sensing by a nanopore
Mark Stamatios Kokoris, Bothell, WA (US); and Robert N. McRuer, Mercer Island, WA (US)
Assigned to Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed by Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed on Oct. 15, 2020, as Appl. No. 17/071,778.
Application 17/071,778 is a continuation of application No. 15/585,029, filed on May 2, 2017, granted, now 10,851,405.
Application 15/585,029 is a continuation of application No. 14/075,837, filed on Nov. 8, 2013, granted, now 9,670,526, issued on Jun. 6, 2017.
Claims priority of provisional application 61/724,821, filed on Nov. 9, 2012.
Prior Publication US 2022/0307067 A1, Sep. 29, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6806 (2018.01); C12Q 1/6869 (2018.01); G01N 33/543 (2006.01)
CPC C12Q 1/6806 (2013.01) [C12Q 1/6869 (2013.01); G01N 33/54373 (2013.01)] 8 Claims
 
1. A method for concentrating a target molecule for nanopore sensing, comprising: capturing the target molecule on a surface, wherein the surface comprises a nanopore and a hydrophobic domain, wherein the target molecule comprises a target portion, a hydrophobic capture element and a leader for interaction with the nanopore, wherein the target portion is an Xpandomer, wherein the Xpandomer is a measurable polymer comprising high signal-to-noise reporters that are separated by approximately 10 nm, wherein the hydrophobic capture element is an aliphatic hydrocarbon, wherein the aliphatic hydrocarbon comprises 5 repeats of a 12 carbon monomer, and wherein the leader is a hydrophobic polymer, wherein the hydrophobic polymer comprises 25 repeats of an ethyl phosphodiester, wherein the hydrophobic capture element is associated with, and capable of movement along, the hydrophobic domain of the surface to bring the leader in proximity with the nanopore; and sensing at least the target portion upon interaction with the nanopore.