US 11,920,166 B2
Variant cannabinoid synthases and methods and uses thereof
Kyle Botsch, San Diego, CA (US); Craig Behnke, San Diego, CA (US); Karla Gonzalez, San Diego, CA (US); Matthew Saunders, San Diego, CA (US); and Michael Mendez, San Diego, CA (US)
Assigned to RENEW BIOPHARMA, INC., San Diego, CA (US)
Filed by RENEW BIOPHARMA, INC., San Diego, CA (US)
Filed on Jul. 20, 2022, as Appl. No. 17/869,608.
Application 17/869,608 is a continuation of application No. 17/049,822, abandoned, previously published as PCT/US2019/028806, filed on Apr. 23, 2019.
Claims priority of provisional application 62/661,524, filed on Apr. 23, 2018.
Prior Publication US 2023/0203455 A1, Jun. 29, 2023
Int. Cl. C12N 9/02 (2006.01)
CPC C12N 9/0004 (2013.01) [C12Y 121/03007 (2015.07)] 20 Claims
 
1. A variant cannabinoid synthase or an active fragment thereof comprising a non-naturally occurring amino acid sequence relative to a wild-type cannabinoid synthase or an active fragment thereof,
wherein the variant cannabinoid synthase is:
(i) a cannabidiolic acid (CBDA) synthase comprising an amino acid sequence having greater than 80% sequence identity to SEQ ID NO: 1043 and an amino acid substitution at at least one amino acid position selected from the group consisting of: H69, C180, M256, R295, Q376, N377, A414, and I445;
(ii) a tetrahydrocannabinolic acid (THCA) synthase comprising an amino acid sequence having greater than 80% sequence identity to SEQ ID NO: 1044 and an amino acid substitution at at least one amino acid position selected from the group consisting of: Q69, G180, I257, K296, K377, K378, V415, and T446; or
(iii) a cannabichromenic acid (CBCA) synthase comprising an amino acid sequence having greater than 80% sequence identity to SEQ ID NO: 1045 and an amino acid substitution at at least one amino acid position selected from the group consisting of: Q69, G180, I257, R296, K377, K378, V415, and T446.