	US 11,919,991 B2
	Enzyme-assisted ATRP procedures
Krzysztof Matyjaszewski, Pittsburgh, PA (US); Alan Enciso Barros, Pittsburgh, PA (US); Liye Fu, Pittsburgh, PA (US); and Alan J. Russell, Pittsburgh, PA (US)
Assigned to Carnegie Mellon University, Pittsburgh, PA (US)
Filed by Carnegie Mellon University, Pittsburgh, PA (US)
Filed on Sep. 12, 2022, as Appl. No. 17/942,952.
Application 17/942,952 is a continuation of application No. 16/520,008, filed on Jul. 23, 2019, granted, now 11,472,894.
Claims priority of provisional application 62/764,221, filed on Jul. 23, 2018.
Prior Publication US 2023/0079313 A1, Mar. 16, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C08F 4/10 (2006.01); C08F 2/16 (2006.01); C08F 4/26 (2006.01); C08K 5/1545 (2006.01); C12P 7/00 (2006.01); C08F 220/28 (2006.01)

CPC C08F 4/10 (2013.01) [C08F 2/16 (2013.01); C08F 4/26 (2013.01); C08K 5/1545 (2013.01); C12P 7/00 (2013.01); C08F 220/28 (2013.01); C08F 220/286 (2020.02); C08F 2438/01 (2013.01); C12Y 101/03004 (2013.01); C12Y 111/01007 (2013.01)]

14 Claims

1. A method for polymerizing free radically copolymerizable monomers, the method comprising combining the copolymerizable monomers with:

(a) a deoxygenated aqueous polymerization medium comprising (i) a transition metal catalyst and (ii) an initiator comprising one or more redox transferable atoms or groups;

(b) an oxygen scavenger comprising glucose and glucose oxidase (GOx); and

(c) a reactive oxygen species (ROS) scavenger comprising one or more of horseradish peroxidase, an α-keto-acid, catalase, and a catalase-like enzyme selected from the group consisting of ascorbate peroxidase, cytochrome C peroxidase, haloperoxidase, hemoprotein, glutathione peroxidase, laccase, monoamine oxidase, cytochrome P450 oxidase, NADPH oxidase, xanthine oxidase, L-gulonolactone oxidase, and superoxide dismutase,

thereby forming a conjugate.