US 11,919,976 B1
Preparation and purification method for cationic quaternary ammonium salt of sodium hyaluronate in homogeneous medium
Jinmao You, Shandong (CN); Lei Liu, Shandong (CN); Chuanli Kang, Shandong (CN); Shuiqiang Yu, Shandong (CN); Deqiang Zheng, Shandong (CN); Qing Li, Shandong (CN); Shuai Du, Shandong (CN); Xinxin Shi, Shandong (CN); Qiang Liu, Shandong (CN); Liwei Tang, Shandong (CN); Shaojie Lian, Shandong (CN); and Meixia Zhang, Shandong (CN)
Assigned to SHANDONG FOCUSFREDA BIOTECH CO., LTD, Shandong (CN)
Filed by SHANDONG FOCUSFREDA BIOTECH CO., LTD, Shandong (CN)
Filed on Apr. 11, 2023, as Appl. No. 18/299,008.
Claims priority of application No. 202211446277.8 (CN), filed on Nov. 18, 2022.
Int. Cl. C08B 37/08 (2006.01)
CPC C08B 37/0072 (2013.01) 8 Claims
 
1. A preparation and purification method for cationic quaternary ammonium salt of sodium hyaluronate in homogeneous medium, comprising the following steps:
adding a homogeneous solvent and a sodium hyaluronate solid to a vessel, heating the vessel until the contents are dissolved to form a homogeneous system, adding an aqueous solution of 2,3-epoxypropyltrimethylammonium chloride to the homogeneous system, then adding a base catalyst, and stirring at a preset temperature to carry out a graft reaction for obtaining a graft product, wherein the 2,3-epoxypropyltrimethylammonium chloride is grafted to the oxygen atom of the hydroxymethyl group of the sodium hyaluronate,
wherein the homogeneous solvent is a mixture of HMF and H2O (HMF-H2O).