US 11,919,971 B2
Aqueous biomolecule coupling on CO2-plasma-activated surfaces
Daniel Christopher Leslie, Brookline, MA (US); Thomas Doyle, Boston, MA (US); Anna Waterhouse, Brookline, MA (US); Melissa Rodas, Boston, MA (US); Alexander L. Watters, North Andover, MA (US); Michael Super, Lexington, MA (US); and Donald E. Ingber, Boston, MA (US)
Assigned to PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US)
Appl. No. 16/302,023
Filed by PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US)
PCT Filed May 16, 2017, PCT No. PCT/US2017/032928
§ 371(c)(1), (2) Date Nov. 15, 2018,
PCT Pub. No. WO2017/201064, PCT Pub. Date Nov. 23, 2017.
Claims priority of provisional application 62/336,940, filed on May 16, 2016.
Prior Publication US 2019/0144567 A1, May 16, 2019
Int. Cl. C07K 17/08 (2006.01); C08L 25/06 (2006.01); C08L 83/04 (2006.01); G01N 33/543 (2006.01); G01N 33/545 (2006.01); C08K 5/05 (2006.01)
CPC C07K 17/08 (2013.01) [C08L 25/06 (2013.01); C08L 83/04 (2013.01); G01N 33/54393 (2013.01); G01N 33/545 (2013.01); C08K 5/05 (2013.01); C08L 2203/02 (2013.01)] 22 Claims
OG exemplary drawing
 
1. A method of making a substrate having an entity attached thereto, the method comprising:
i) contacting the substrate with a CO2 plasma to form a modified substrate comprising a plasma-generated-moiety (PGM) comprising a carboxylic acid group, wherein the substrate comprises polysulfone (PS), polyarylethersulfone (PAES) or polyethersulfone (PES); and
ii) contacting the entity with the modified substrate under conditions sufficient for attachment of the entity to the modified substrate; wherein step ii) contacting is performed for about 4-16 hours;
thereby making a substrate having the entity attached thereto,
provided that
the entity comprises a lectin; and
provided that:
the modified substrate is not contacted with a crosslinking moiety of derivatized with a crosslinking moiety, prior to attachment of the entity;
the modified substrate is not contacted with an organic solvent prior to attachment of the entity; and
the entity is contacted with the modified substrate under aqueous conditions.