US 11,919,969 B2
Bispecific antibodies for factor IX and factor X
Wei Wang, Cambridge (GB); E-Chiang Lee, Cambridge (GB); John Kenneth Blackwood, Cambridge (GB); and Roberto Magliozzi, Cambridge (GB)
Assigned to KYMAB LIMITED, Cambridge (GB)
Appl. No. 16/625,109
Filed by KYMAB LIMITED, Cambridge (GB)
PCT Filed Jun. 22, 2018, PCT No. PCT/EP2018/066836
§ 371(c)(1), (2) Date Dec. 20, 2019,
PCT Pub. No. WO2018/234575, PCT Pub. Date Dec. 27, 2018.
Claims priority of application No. 1709970.6 (GB), filed on Jun. 22, 2017.
Prior Publication US 2020/0148787 A1, May 14, 2020
Int. Cl. C07K 16/36 (2006.01); A61P 7/04 (2006.01); C07K 16/40 (2006.01)
CPC C07K 16/36 (2013.01) [A61P 7/04 (2018.01); C07K 16/40 (2013.01); C07K 2317/21 (2013.01); C07K 2317/31 (2013.01); C07K 2317/55 (2013.01); C07K 2317/56 (2013.01); C07K 2317/64 (2013.01); C07K 2317/75 (2013.01); C07K 2317/76 (2013.01); C07K 2317/92 (2013.01)] 9 Claims
 
1. A bispecific antigen-binding molecule comprising:
a FIXa binding polypeptide arm comprising a FIXa binding site, and
a FX binding polypeptide arm comprising a FX binding site
wherein the FIXa binding site is provided by a set of complementarity determining regions (CDRs) in the FIXa binding polypeptide arm, the set of CDRs comprising HCDR1, HCDR2 and HCDR3, wherein HCDR1 is SEQ ID NO: 1, HCDR2 is SEQ ID NO: 2 and HCDR3 is SEQ ID NO: 171, and LCDR1, LCDR2 and LCDR3, wherein LCDR1 is SEQ ID NO: 6, LCDR2 is SEQ ID NO: 7 and LCDR3 is SEQ ID NO: 8;
wherein the FX binding site is provided by a set of CDRs in the FX binding polypeptide arm, the set of CDRs selected from the group consisting of:
(i) HCDR1 is SEQ ID NO: 67, HCDR2 is SEQ ID NO: 68 and HCDR3 is SEQ ID NO: 69, and LCDR1 is SEQ ID NO: 72, LCDR2 is SEQ ID NO: 73 and LCDR3 is SEQ ID NO: 74;
(ii) HCDR1 is SEQ ID NO: 57, HCDR2 is SEQ ID NO: 58 and HCDR3 is SEQ ID NO: 59, and LCDR1 is SEQ ID NO: 62, LCDR2 is SEQ ID NO: 63 and LCDR3 is SEQ ID NO: 64;
(iii) HCDR1 is SEQ ID NO: 96, HCDR2 is SEQ ID NO: 97 and HCDR3 is SEQ ID NO: 98, and LCDR1 is SEQ ID NO: 101, LCDR2 is SEQ ID NO: 92 and LCDR3 is SEQ ID NO: 102; and
(iv) HCDR1 is SEQ ID NO: 77, HCDR2 is SEQ ID NO: 78 and HCDR3 is SEQ ID NO: 79, and LCDR1 is SEQ ID NO: 62, LCDR2 is SEQ ID NO: 82 and LCDR3 is SEQ ID NO: 83.