US 11,919,000 B2
Methods and systems for microfluidic screening
Devon Cayer, Del Mar, CA (US); Andrew MacConnell, Del Mar, CA (US); Pavel Chubukov, Del Mar, CA (US); Ramesh Ramji, Del Mar, CA (US); and Sean Stromberg, Del Mar, CA (US)
Assigned to 1859, Inc., San Diego, CA (US)
Filed by 1859, Inc., Del Mar, CA (US)
Filed on Oct. 9, 2020, as Appl. No. 17/067,534.
Claims priority of provisional application 62/954,348, filed on Dec. 27, 2019.
Claims priority of provisional application 62/913,624, filed on Oct. 10, 2019.
Prior Publication US 2021/0106996 A1, Apr. 15, 2021
Int. Cl. B01L 3/00 (2006.01); C12N 15/10 (2006.01); C12Q 1/6844 (2018.01); C12Q 1/686 (2018.01); C12Q 1/6876 (2018.01); C40B 20/04 (2006.01); C40B 30/08 (2006.01); C40B 50/06 (2006.01); C40B 50/16 (2006.01)
CPC B01L 3/502761 (2013.01) [C12N 15/1093 (2013.01); C12Q 1/6844 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6876 (2013.01); C40B 50/06 (2013.01); C40B 50/16 (2013.01); B01L 2200/0652 (2013.01); B01L 2400/0403 (2013.01); C40B 20/04 (2013.01); C40B 30/08 (2013.01)] 19 Claims
 
1. A method for screening encoded effectors to identify ion channel modulators, the method comprising:
(a) providing an encapsulation comprising:
(i) a cell expressing an ion channel protein;
(ii) a set of voltage sensor probes; and
(iii) an encoded effector and its corresponding encoding;
(b) stimulating the ion channel protein of the cell; and
(c) detecting a signal from at least one member of the set of voltage sensor probes,
wherein the encoded effector is identified as an ion channel modulator based on a difference in the signal relative to a signal from a different encapsulation.