US 11,918,822 B2
Compositions and methods for enhancing the biological response to chemical agents and physical stimuli
Clinton Rubin, Port Jefferson, NY (US); and Janet Rubin, Chapel Hill, NC (US)
Assigned to THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, Albany, NY (US); and THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL, Chapel Hill, NC (US)
Filed by The Research Foundation for the State University of New York, Albany, NY (US); and The University of North Carolina at Chapel Hill, Chapel Hill, NC (US)
Filed on Oct. 19, 2022, as Appl. No. 17/969,131.
Application 17/969,131 is a continuation of application No. 17/354,112, filed on Jun. 22, 2021, abandoned.
Application 17/354,112 is a continuation of application No. 16/040,659, filed on Jul. 20, 2018, granted, now 11,040,215, issued on Jun. 22, 2021.
Application 16/040,659 is a continuation of application No. 13/879,551, granted, now 10,029,089, issued on Jul. 24, 2018, previously published as PCT/US2011/056289, filed on Oct. 14, 2011.
Claims priority of provisional application 61/393,812, filed on Oct. 15, 2010.
Prior Publication US 2023/0173292 A1, Jun. 8, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A61N 2/02 (2006.01); A61M 5/00 (2006.01); A61N 1/05 (2006.01); C12N 13/00 (2006.01); A61M 31/00 (2006.01); A61N 1/32 (2006.01); A61N 2/00 (2006.01); A61N 5/06 (2006.01)
CPC A61N 2/02 (2013.01) [A61M 5/00 (2013.01); A61N 1/05 (2013.01); C12N 13/00 (2013.01); A61M 31/002 (2013.01); A61N 1/326 (2013.01); A61N 2/00 (2013.01); A61N 5/062 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method of enhancing a cell response to a high frequency, low magnitude physical stimulus, the method comprising:
providing an ex vivo cell;
providing a device configured to deliver the high frequency, low magnitude physical stimulus toward the ex vivo cell;
exposing the ex vivo cell to the physical stimulus, wherein the physical stimulus is applied to the ex vivo cell for a first session comprising about one second to about one hour;
providing for a refractory period wherein the same ex vivo cell is free of the physical stimulus for about the next 1-3 hours; and
exposing the same ex vivo cell to the physical stimulus for a second session comprising about one second to about one hour immediately after the about 1-3 hours of being free of the physical stimulus, wherein the response is enhanced relative to an ex vivo cell receiving the same stimulus without the refractory period, wherein a response by the ex vivo cell to the physical stimulus is an increase in cellular proliferation.