US 12,241,899 B2
Systems and methods for sample preparation, data generation, and protein corona analysis
William Manning, Redwood City, CA (US); Young Kim, Union City, CA (US); Brandon Kwan-Leong, Daly City, CA (US); Hope Liou, Pleasanton, CA (US); Xiaoyan Zhao, Foster City, CA (US); Daniel Hornburg, Foster City, CA (US); and Martin Goldberg, Saratoga, CA (US)
Assigned to Seer, Inc., Redwood City, CA (US)
Filed by Seer, Inc., Redwood City, CA (US)
Filed on Mar. 3, 2023, as Appl. No. 18/178,288.
Application 18/178,288 is a continuation of application No. 17/216,523, filed on Mar. 29, 2021, granted, now 11,630,112.
Application 17/216,523 is a continuation of application No. PCT/US2020/044908, filed on Aug. 4, 2020.
Claims priority of provisional application 62/883,107, filed on Aug. 5, 2019.
Prior Publication US 2023/0204596 A1, Jun. 29, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/68 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); G01N 1/34 (2006.01); G01N 1/44 (2006.01); G01N 33/543 (2006.01); B82Y 5/00 (2011.01); B82Y 25/00 (2011.01); B82Y 30/00 (2011.01); B82Y 35/00 (2011.01); B82Y 40/00 (2011.01)
CPC G01N 33/6848 (2013.01) [B01L 3/5085 (2013.01); B01L 7/00 (2013.01); G01N 1/34 (2013.01); G01N 1/44 (2013.01); G01N 33/54326 (2013.01); G01N 33/54346 (2013.01); B01L 2400/043 (2013.01); B82Y 5/00 (2013.01); B82Y 25/00 (2013.01); B82Y 30/00 (2013.01); B82Y 35/00 (2013.01); B82Y 40/00 (2013.01)] 18 Claims
OG exemplary drawing
 
1. An automated system for distinguishing states of a biological sample using a plurality of magnetic particles, the system comprising:
(a) a fluid transfer unit comprising a multichannel fluid transfer instrument for transferring fluids between units within the system, the multichannel fluid transfer instrument comprising a plurality of pipettes;
(b) a sensor array comprising a magnetic support and a magnetic sensor array, wherein the magnetic sensor array is removably coupled to the magnetic support and comprises a plurality of partitions that comprises (i) a biological sample, (ii) a diluent comprising a buffer, and (iii) a plurality of magnetic particles, wherein the plurality of magnetic particles comprise a carboxyl functionalized silica surface, and wherein the plurality of magnetic particles are configured to bind to at least 300 different proteins from the biological sample;
(c) a reagent storage unit comprising supports for storing a plurality of reagents, wherein the plurality of reagents comprise a buffer, a wash solution, a resuspension solution, and a denaturing solution comprising a protease, wherein the protease comprises trypsin;
(d) a waste unit comprising supports for storing a reagent to be disposed of;
(e) a consumable storage comprising supports for storing consumables for use by the multichannel fluid transfer instrument, the consumables comprising a plurality of pipette tips; and
(f) a control unit comprising one or more processors, wherein the one or more processors comprises a program to perform steps comprising:
i. contacting the biological sample with at least one partition of the plurality of partitions of the magnetic sensor array;
ii. incubating the biological sample with the plurality of magnetic particles of the magnetic sensor array; and
iii. preparing a population of proteins for mass spectrometry by adding the resuspension solution and the denaturing solution to the partition to desorb and digest a plurality of proteins in the biological sample, thereby generating the population of proteins.