US 12,241,892 B2
In situ serial dilution method
Emmanuel Guignet, Cressier FR (CH)
Assigned to BIO-RAD EUROPE GMBH, Basel (CH)
Appl. No. 16/626,584
Filed by BIO-RAD EUROPE GMBH, Basel (CH)
PCT Filed Jun. 22, 2018, PCT No. PCT/IB2018/000768
§ 371(c)(1), (2) Date Dec. 26, 2019,
PCT Pub. No. WO2019/002935, PCT Pub. Date Jan. 3, 2019.
Claims priority of provisional application 62/524,832, filed on Jun. 26, 2017.
Prior Publication US 2020/0116710 A1, Apr. 16, 2020
Int. Cl. G01N 33/543 (2006.01)
CPC G01N 33/54306 (2013.01) 20 Claims
OG exemplary drawing
 
1. A method of determining a concentration of an analyte in a sample by in situ serial dilution, the method comprising:
providing an immunodiagnostic test card, the test card comprising a substantially flat support that supports a plurality of vertically disposed microtubes, each microtube having an incubation chamber for receiving the sample and a reaction chamber comprising a separation matrix;
adding a diluent to at least one of the incubation chambers;
serially diluting the sample directly into each of the incubation chambers having diluent therein;
adding a reagent to each of the incubation chambers having diluent and/or sample therein, wherein the reagent is capable of complexing with the analyte to form a reagent-analyte complex;
exposing the mixture of sample and reagent to sedimentation by gravitation and/or centrifugation; and
determining the concentration of the analyte by determining a highest dilution of the sample at which a reagent-analyte complex is detected on or within the separation matrix.