US 12,241,891 B2
Multivalent binding composition for nucleic acid analysis
Michael Previte, San Diego, CA (US); Molly He, San Diego, CA (US); Junhua Zhao, San Diego, CA (US); Hui Zhen Mah, San Diego, CA (US); Chunhong Zhou, San Diego, CA (US); Sinan Arslan, San Diego, CA (US); Matthew Kellinger, San Diego, CA (US); Lorenzo Berti, San Diego, CA (US); and Steve Xiangling Chen, San Diego, CA (US)
Assigned to ELEMENT BIOSCIENCES, INC., San Diego, CA (US)
Filed by Element Biosciences, Inc., San Diego, CA (US)
Filed on Feb. 18, 2022, as Appl. No. 17/675,154.
Application 17/675,154 is a continuation of application No. 17/356,929, filed on Jun. 24, 2021, granted, now 11,287,422.
Application 17/356,929 is a continuation of application No. PCT/US2020/052305, filed on Sep. 23, 2020.
Claims priority of provisional application 62/904,623, filed on Sep. 23, 2019.
Prior Publication US 2022/0170919 A1, Jun. 2, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/53 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6874 (2018.01); G01N 33/58 (2006.01)
CPC G01N 33/5308 (2013.01) [C12Q 1/6874 (2013.01); G01N 33/582 (2013.01)] 20 Claims
 
1. A method for identifying at least a portion of a sub-cellular component within a cell or tissue in situ, the method comprising:
(a) detecting a signal from a binding complex between said sub-cellular component and a detectable polymer-nucleotide conjugate within said cell or said tissue, wherein said detectable polymer-nucleotide conjugate comprises at least two nucleotides that are transiently interacting with at least two of said sub-cellular components; and
(b) processing at least said signal detected in (a) to identify said at least said portion of said sub-cellular component.