US 12,241,859 B2
Polymer-coating of electrodes for sensor devices
Alexey Tarasov, Neckargemuend (DE); Nesha Andoy, Heidelberg (DE); Marcin Filipiak, Dossenheim (DE); Oscar Gutierrez-Sanz, Heidelberg (DE); and Natalie Haustein, Dossenheim (DE)
Assigned to Roche Diagnostics Operations, Inc., Indianapolis, IN (US)
Filed by Roche Diagnostics Operations, Inc., Indianapolis, IN (US)
Filed on Aug. 21, 2019, as Appl. No. 16/546,452.
Application 16/546,452 is a continuation of application No. PCT/EP2018/054280, filed on Feb. 21, 2018.
Claims priority of application No. 17157373 (EP), filed on Feb. 22, 2017.
Prior Publication US 2020/0033291 A1, Jan. 30, 2020
Int. Cl. G01N 27/414 (2006.01); G01N 33/543 (2006.01)
CPC G01N 27/4145 (2013.01) [G01N 33/5438 (2013.01)] 3 Claims
 
1. A method for manufacturing a functionalized surface on an electrode comprising the steps of:
a) applying to the electrode a linker and at least one polymer capable of mediating a salting-out effect as a homogenous solution under conditions which allow for covalent or non-covalent immobilization of the linker and the at least one polymer capable of mediating a salting-out effect on the surface of the electrode, wherein the linker has the following structure:
A-B—C
wherein A is a first functional group selected from the group consisting of thiol, silane, phosphonic acid, aromatic molecules, carboxyl, amine, NHS ester, and maleimide, wherein B is a low molecular weight (MW) polymer having a MW of 0.1 to 1 kDa, and wherein C is a second functional group selected from the group consisting of carboxyl, amine, NHS ester, and maleimide; and
b) applying at least one detection agent to the electrode upon immobilization of the linker and the at least one polymer capable of mediating a salting-out effect under conditions which allow for covalent or non-covalent attachment of the at least one detection agent to the electrode via the immobilized linker; and
wherein the conditions allow for distributing the at least one polymer capable of mediating a salting-out effect and the at least one detection agent on the surface of the electrode such that the at least one detection agent is present in equal amounts per surface area throughout the electrode surface and the at least one polymer capable of mediating a salting-out effect is arranged around the detection agent present in an amount
i) allowing the reduction of ionic strength of a fluid sample in proximity to the detection agent, and
ii) allowing for binding an analyte comprised in the fluid sample;
wherein the at least one polymer capable of mediating a salting-out effect has a molecular weight (MW) between 1 and 50 kDa and the size of the polymer capable of mediating a salting-out effect is larger or equal to the size of the at least one detection agent;
wherein the at least one polymer capable of mediating a salting-out effect is of the same class of polymers as B of the linker; and
wherein B of the linker and the at least one polymer capable of mediating a salting-out effect are selected from the group consisting of poly(ethylene glycol) (PEG), poly(ethylene oxide), poly(propylene glycol), polyglycerol, polyacrylamide (PAM), polyethyleneimine (PEI), polymethacrylate, poly(vinyl alcohol) (PVA), poly(vinylpyrrolidone) (PVP), and copolymers of the aforementioned polymers.