| CPC G01N 21/6428 (2013.01) [G01N 21/645 (2013.01); G01N 2021/6439 (2013.01); G01N 2021/6482 (2013.01); G01N 2021/6491 (2013.01)] | 23 Claims |

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1. An instrument for performing an assay, comprising:
a probe;
a support structure positioned proximal to the probe, wherein a container for one or more fluids is positioned on the support structure; and
an optical detector configured to detect an optical signal, wherein:
a reagent is immobilized on the probe,
the probe is capable of being translated by the instrument between a plurality of locations at which a plurality of fluids are positioned, and
the optical signal comprises:
light that has been emitted and/or transmitted from a species immobilized on the probe,
light that has been emitted, transmitted, reflected, and/or scattered from a species generated from a species immobilized on the probe,
the absence of light due to absorption of light by a species immobilized on the probe, and/or
the absence of light due to absorption of light by a species generated from a species immobilized on the probe.
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