US 12,241,119 B2
Polymerization of nucleic acids using proteins having low isoelectric points
Jonathan Wang, Mountain View, CA (US); and David Dupont, Redwood City, CA (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US)
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed on May 25, 2022, as Appl. No. 17/824,637.
Application 17/824,637 is a division of application No. 16/212,011, filed on Dec. 6, 2018, granted, now 11,365,443.
Application 16/212,011 is a division of application No. 15/397,938, filed on Jan. 4, 2017, granted, now 10,378,050, issued on Aug. 13, 2019.
Application 15/397,938 is a division of application No. 14/124,667, granted, now 9,567,628, issued on Feb. 14, 2017, previously published as PCT/US2012/041687, filed on Jun. 8, 2012.
Claims priority of provisional application 61/494,797, filed on Jun. 8, 2011.
Prior Publication US 2022/0307072 A1, Sep. 29, 2022
Int. Cl. C12Q 1/68 (2018.01); C12N 9/12 (2006.01); C12N 15/10 (2006.01); C12Q 1/6844 (2018.01); C12Q 1/686 (2018.01)
CPC C12Q 1/686 (2013.01) [C12N 9/1252 (2013.01); C12N 15/10 (2013.01); C12Q 1/6846 (2013.01); C12Y 207/07007 (2013.01)] 8 Claims
 
1. A kit comprising:
a storage or reaction composition including a polymerase and at least one protein having a low isoelectric point selected from the group consisting of b-lactoglobulin, apomyoglobin, a-lactoalbumin, apotransferrin, a-acid glycoprotein, and b-casein, said storage or reaction composition being substantially free of detergent.