US 12,241,118 B2
Assay for the rapid detection of nucleic acids via a modified lamp reaction coupled with colorimetric reporter utilizing a gold nanoparticle : peptide nucleic acid (AuNp-PNA) probe system
Paul Mann, Marquette, MI (US); and Matthew Jennings, Marquette, MI (US)
Filed by Paul Mann, Marquette, MI (US); and Matthew Jennings, Marquette, MI (US)
Filed on Aug. 22, 2022, as Appl. No. 17/821,277.
Application 17/821,277 is a continuation of application No. 16/706,243, filed on Dec. 6, 2019, abandoned.
Claims priority of provisional application 62/776,104, filed on Dec. 6, 2018.
Prior Publication US 2023/0227898 A1, Jul. 20, 2023
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6816 (2018.01); C12Q 1/6853 (2018.01); C12Q 1/6869 (2018.01)
CPC C12Q 1/6853 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6869 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01)] 10 Claims
 
1. A method of rapidly performing a diagnostic assay for detection of nucleic acids (DNA/RNA), comprising:
performing a LAMP reaction with a Loop Mediated Isothermal Amplification (LAMP) device to process a test solution and detect at least one specific nucleic acid sequence in said test solution that defines a target dependent sequence of a nucleic acid that is targeted for detection by said rapid diagnostic assay, wherein said LAMP reaction performs isothermal amplification of said target dependent sequence of the nucleic acid, said LAMP reaction using a plurality of primers to detect target sequences wherein one or more of said primers have a poly T linker replaced with a peptide nucleic acid (PNA) sequence to form a PNA/DNA hybrid that corresponds to and binds with said target dependent sequence of said nucleic acids; and
providing a colorimetric reporter system using gold nanoparticles (AuNP) aggregatable with a peptide nucleic acid (PNA); and
using said colorimetric reporter system to identify and detect said target dependent sequence of said nucleic acid sequences and identify the presence of said target dependent sequence.