US 12,241,106 B2
Biosurfactant-producing recombinant microorganism
Tomotake Morita, Tsukuba (JP); Azusa Saika, Tsukuba (JP); Hideaki Koike, Tsukuba (JP); Tokuma Fukuoka, Tsukuba (JP); Dai Kitamoto, Tsukuba (JP); Tomohiro Sugahara, Tsuruga (JP); Shuhei Yamamoto, Osaka (JP); and Atsushi Sogabe, Osaka (JP)
Assigned to NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY, Tokyo (JP); and TOYOBO CO., LTD., Osaka (JP)
Appl. No. 17/626,475
Filed by NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY, Tokyo (JP); and TOYOBO CO., LTD., Osaka (JP)
PCT Filed Jul. 8, 2020, PCT No. PCT/JP2020/026760
§ 371(c)(1), (2) Date Jan. 11, 2022,
PCT Pub. No. WO2021/010264, PCT Pub. Date Jan. 21, 2021.
Claims priority of application No. 2019-133055 (JP), filed on Jul. 18, 2019.
Prior Publication US 2022/0282296 A1, Sep. 8, 2022
Int. Cl. C12P 19/44 (2006.01); C12N 9/20 (2006.01); C12N 15/52 (2006.01); C12N 15/81 (2006.01); C40B 40/08 (2006.01)
CPC C12P 19/44 (2013.01) [C12N 9/20 (2013.01); C12N 15/52 (2013.01); C12N 15/815 (2013.01); C12Y 301/01003 (2013.01); C40B 40/08 (2013.01)] 10 Claims
 
1. A mannosylerythritol-lipid-producing microorganism transformed with an expression vector containing a gene that encodes a lipase under the control of E5Pgap promoter comprising the nucleotide sequence of SEQ ID NO: 1 or E5Ptef promoter comprising the nucleotide sequence of SEQ ID NO: 2, wherein the lipase comprises an amino acid sequence that is at least 80% identical to the amino acid sequence of any of SEQ ID NO: 5 to 13.