US 12,241,092 B2
Products of manufacture and methods to enrich for and isolate viruses or phages capable of targeting specific microbes
Forest Rohwer, San Diego, CA (US)
Assigned to San Diego State University (SDSU) Foundation, San Diego, CA (US)
Appl. No. 16/761,037
Filed by San Diego State University (SDSU) Foundation, San Diego, CA (US)
PCT Filed Nov. 2, 2018, PCT No. PCT/US2018/058879
§ 371(c)(1), (2) Date May 1, 2020,
PCT Pub. No. WO2019/090034, PCT Pub. Date May 9, 2019.
Claims priority of provisional application 62/581,309, filed on Nov. 3, 2017.
Prior Publication US 2021/0180030 A1, Jun. 17, 2021
Int. Cl. C12N 7/00 (2006.01); A01N 63/40 (2020.01); A61K 35/76 (2015.01); C12N 7/02 (2006.01)
CPC C12N 7/00 (2013.01) [A01N 63/40 (2020.01); A61K 35/76 (2013.01); C12N 7/02 (2013.01); C12N 2795/00021 (2013.01); C12N 2795/00031 (2013.01); C12N 2795/00032 (2013.01); C12N 2795/00051 (2013.01)] 22 Claims
 
1. An in vitro method for generating a virus or a bacteriophage having a modified phenotype, comprising:
(a) generating in vitro a first microbial niche or microenvironment having one or a plurality of microbes, or having a community of microbes or having a complex community of different microbes;
(b) inoculating in vitro into the first microbial niche or microenvironment a virus or a bacteriophage or a plurality of viruses or bacteriophages from a second microbial niche or microenvironment, thereby generating a third microbial niche or microenvironment;
(c) culturing in vitro the third microbial niche or microenvironment for multiple rounds of culturing, wherein each additional round of culturing is carried out under culture conditions that are different from culture conditions used in a previous round of culturing, and after the multiple rounds of culturing the virus and/or bacteriophage having the modified phenotype is generated; and
(d) isolating the virus or bacteriophage having the modified phenotype from the cultured third microbial niche or microenvironment after the multiple rounds of culturing.