US 12,241,091 B2
Method of making exosomes from tumor cell/antigen presenting cell hybrid cells
Guanghui Ma, Beijing (CN); Wei Wei, Beijing (CN); Shuang Wang, Beijing (CN); Shuang Qing, Beijing (CN); and Jianghua Wang, Beijing (CN)
Assigned to INSTITUTE OF PROCESS ENGINEERING, CHINESE ACADEMY OF SCIENCES, Beijing (CN)
Appl. No. 17/269,897
Filed by INSTITUTE OF PROCESS ENGINEERING, CHINESE ACADEMY OF SCIENCES, Beijing (CN)
PCT Filed Aug. 16, 2019, PCT No. PCT/CN2019/101079
§ 371(c)(1), (2) Date Feb. 19, 2021,
PCT Pub. No. WO2020/038299, PCT Pub. Date Feb. 27, 2020.
Claims priority of application No. 201810946746.X (CN), filed on Aug. 20, 2018.
Prior Publication US 2021/0324342 A1, Oct. 21, 2021
Int. Cl. C12N 5/00 (2006.01); A61K 35/15 (2015.01); A61K 35/17 (2015.01); A61K 39/00 (2006.01); A61P 35/00 (2006.01); C07K 16/28 (2006.01); C12N 5/0786 (2010.01); C12N 5/16 (2006.01)
CPC C12N 5/163 (2013.01) [A61K 35/15 (2013.01); A61K 35/17 (2013.01); A61K 39/0011 (2013.01); A61P 35/00 (2018.01); C07K 16/2818 (2013.01); C07K 16/2827 (2013.01); C12N 5/0645 (2013.01); A61K 2039/5154 (2013.01); A61K 2039/572 (2013.01)] 3 Claims
 
1. A method of preparing an exosome, the method comprising:
a) extracting nuclei from isolated tumor cells;
b) adding the nuclei to antigen presenting cells (APCs) in culture such that hybrid cells are obtained;
c) incubating the hybrid cells obtained in step b);
d) collecting supernatant from the culture medium comprising the hybrid cells incubated in step c);
e) isolating exosomes from the supernatant.