US 12,241,090 B2
Methods and systems for converting precursor cells into gastric tissues through directed differentiation
James Macormack Wells, Cincinnati, OH (US); and Kyle William McCracken, Cincinnati, OH (US)
Assigned to Children's Hospital Medical Center, Cincinnati, OH (US)
Filed by CHILDREN'S HOSPITAL MEDICAL CENTER, Cincinnati, OH (US)
Filed on Jul. 1, 2021, as Appl. No. 17/305,196.
Application 17/305,196 is a continuation of application No. 16/188,597, filed on Nov. 13, 2018, granted, now 11,053,477.
Application 16/188,597 is a continuation of application No. 15/312,939, granted, now 10,174,289, issued on Jan. 8, 2019, previously published as PCT/US2015/032626, filed on May 27, 2015.
Claims priority of provisional application 62/003,719, filed on May 28, 2014.
Prior Publication US 2022/0056420 A1, Feb. 24, 2022
Int. Cl. C12N 5/071 (2010.01)
CPC C12N 5/0697 (2013.01) [C12N 5/0679 (2013.01); C12N 2501/11 (2013.01); C12N 2501/113 (2013.01); C12N 2501/115 (2013.01); C12N 2501/119 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2501/385 (2013.01); C12N 2501/415 (2013.01); C12N 2501/727 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A human gastric organoid (hGO), characterized by:
a) a lumen and glandular epithelium;
b) MUC5AC+ surface mucous cells that secrete mucus into the lumen and TFF2+ mucous gland cells; and
c) chromogranin A (CHGA)-positive endocrine cells.