	US 12,241,083 B2
	Macs-based purification of stem cell-derived retinal pigment epithelium
Nathan Meyer, Madison, WI (US); Lucas Chase, Madison, WI (US); and Casey Stankewicz, Madison, WI (US)
Assigned to FUJIFILM Cellular Dynamics, Inc., Madison, WI (US)
Filed by FUJIFILM Cellular Dynamics, Inc., Madison, WI (US)
Filed on Oct. 4, 2021, as Appl. No. 17/493,533.
Application 17/493,533 is a continuation of application No. 15/258,744, filed on Sep. 7, 2016, granted, now 11,162,070.
Claims priority of provisional application 62/215,272, filed on Sep. 8, 2015.
Prior Publication US 2022/0033770 A1, Feb. 3, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/00 (2006.01); C12N 5/079 (2010.01)

CPC C12N 5/0081 (2013.01) [C12N 5/0621 (2013.01); C12N 2506/03 (2013.01)]

19 Claims

1. A method for enriching for RPE cells from a starting population of RPE cells, the method comprising depleting CD56+ cells from the starting RPE cell population to provide the RPE-enriched cell population, wherein at least 90% of the cells in the RPE-enriched cell population are PMEL17+ and at least 90% of the cells in the RPE-enriched population are TYRP1+.