	US 12,241,070 B2
	Serine recombinases mediating stable integration into plant genomes
Shiv B. Tiwari, Blacksburg, VA (US); Arianne Tremblay, Blacksburg, VA (US); Jonathan Carson, Blacksburg, VA (US); Sekhar Boddupalli, Blacksburg, VA (US); Rio Stamler, Blacksburg, VA (US); Amanda Edwards, Blacksburg, VA (US); and Kornelie Frech, Blacksburg, VA (US)
Assigned to GREENVENUS, LLC, Davis, CA (US)
Appl. No. 17/290,114
Filed by GREENVENUS, LLC, Davis, CA (US)
PCT Filed Oct. 31, 2019, PCT No. PCT/US2019/059107 § 371(c)(1), (2) Date Apr. 29, 2021, PCT Pub. No. WO2020/092733, PCT Pub. Date May 7, 2020.
Claims priority of provisional application 62/914,633, filed on Oct. 14, 2019.
Claims priority of provisional application 62/913,318, filed on Oct. 10, 2019.
Claims priority of provisional application 62/849,368, filed on May 17, 2019.
Claims priority of provisional application 62/754,745, filed on Nov. 2, 2018.
Prior Publication US 2021/0395761 A1, Dec. 23, 2021
Int. Cl. C12N 15/82 (2006.01); C12N 9/22 (2006.01)

CPC C12N 15/8213 (2013.01) [C12N 9/22 (2013.01); C12N 15/8241 (2013.01)]

21 Claims

1. A method for incorporating an exogenous DNA into a plant genome, comprising:

co-transfecting a plant cell with:

(a) an exogenous DNA comprising an attP or attB site and

(b) a polynucleotide encoding an SPβc2 serine recombinase comprising a nucleotide sequence of SEQ ID NO:36, or a nucleotide sequence having at least 95% sequence identity to SEQ ID NO:36, operably linked to a promoter that is active in the plant;

selecting for integration of the exogenous DNA into the plant cell genome at a plant att pseudosite comprising a core sequence of SEQ ID NO:99 or SEQ ID NO:101 or a consensus sequence of SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO: 100, SEQ ID NO: 130, or SEQ ID NO: 131; and

growing the plant cell.