	US 12,241,058 B2
	Recombinant microbial system for directed evolution of glycocins and method of preparation thereof
Alka Rao, Chandigarh (IN); and Pravinkumar Vishavanath Choudhary, Chandigarh (IN)
Assigned to COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH, New Delhi (IN)
Appl. No. 17/272,221
Filed by COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH, New Delhi (IN)
PCT Filed Aug. 28, 2019, PCT No. PCT/IN2019/050623 § 371(c)(1), (2) Date Feb. 26, 2021, PCT Pub. No. WO2020/044371, PCT Pub. Date Mar. 5, 2020.
Claims priority of application No. 201811032280 (IN), filed on Aug. 29, 2018.
Prior Publication US 2021/0395728 A1, Dec. 23, 2021
Int. Cl. C12N 15/10 (2006.01); C12N 15/70 (2006.01); C12P 19/18 (2006.01); C12P 19/44 (2006.01)

CPC C12N 15/1058 (2013.01) [C12N 15/1082 (2013.01); C12N 15/70 (2013.01); C12P 19/18 (2013.01); C12P 19/44 (2013.01)]

16 Claims

1. A recombinant microbial system for synthesis of libraries of O- and S-linked neo-glycocins, and directed evolution thereof, wherein the recombinant microbial system comprises:

a) a cloning vector pRSF Duet-1SapI having the nucleotide sequence as set forth in SEQ ID NO: 68;

b) a gene cassette A comprising a DNA sequence encoding glycosyltransferase, and a DNA sequence encoding an acceptor sequence having a polynucleotide sequence as set forth in sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5; and

c) a host cell.