US 12,241,050 B2
Perfusion filter assembly for increased cross flow
Ralph Stankowski, Westborough, MA (US)
Assigned to Global Life Sciences Solutions USA, LLC, Marlborough, MA (US)
Filed by Global Life Sciences Solutions USA LLC, Marlborough, MA (US)
Filed on Sep. 1, 2021, as Appl. No. 17/464,599.
Prior Publication US 2023/0066761 A1, Mar. 2, 2023
Int. Cl. C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 3/00 (2006.01)
CPC C12M 23/14 (2013.01) [C12M 23/26 (2013.01); C12M 23/48 (2013.01); C12M 25/02 (2013.01); C12M 37/04 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A bioreactor bag comprising:
a flexible bag having an inner surface and an outer surface;
a perfusion filter assembly, the filter assembly comprising:
a horizontally oriented support plate with an opening proximate the top that is adapted to be connected with a tube for retrieving filtrate;
a filter membrane comprising a porous filter region, wherein the filter membrane is bonded proximate the bottom of the support plate in a peripheral region of the filter membrane; and
at least one support structure abutting the bottom of the filter membrane adapted to prevent or reduce sagging of the porous filter region during use;
wherein the filter assembly is mounted in the flexible bag with an exposed surface of the porous filter region facing the bottom of the flexible bag and a plurality of stand-offs contact the inner surface of the bag and filter assembly that are adapted to assure that there is a distance between the filter assembly and the inner surface of the flexible bag for fluid flow between the filter membrane and the inner surface of the flexible bag.