US 12,240,900 B1
Nucleic acids, vectors, and cells that encode antibodies and other proteins that bind folate receptor alpha
Seah Lim, Wilmington, DE (US)
Assigned to MEDICOVESTOR, INC., Wilmington, DE (US)
Filed by Medicovestor, Inc., Wilmington, DE (US)
Filed on Feb. 2, 2024, as Appl. No. 18/431,764.
Int. Cl. C07K 16/28 (2006.01)
CPC C07K 16/28 (2013.01) [C07K 2317/21 (2013.01); C07K 2317/55 (2013.01); C07K 2317/92 (2013.01)] 15 Claims
 
1. A recombinant nucleic acid, comprising:
a nucleotide sequence that encodes a protein that comprises an antigen-binding region, wherein:
the antigen-binding region comprises a first variable domain and a second variable domain;
the first variable domain comprises a VH CDR1 region including an amino acid sequence that is identical to at least 9 consecutive amino acids of SEQ ID NO: 5, a VH CDR2 region including an amino acid sequence that is identical to at least 16 consecutive amino acids of SEQ ID NO: 6, and a VH CDR3 region including an amino acid sequence that is identical to at least 10 consecutive amino acids of SEQ ID NO: 7;
the second variable domain comprises a VL CDR1 region including an amino acid sequence that is identical to at least 10 consecutive amino acids of SEQ ID NO: 8, a VL CDR2 region including an amino acid sequence that is identical to at least 6 consecutive amino acids of SEQ ID NO: 9, and a VL CDR3 region including an amino acid sequence that is identical to at least 9 consecutive amino acids of SEQ ID NO: 10; and
wherein the first variable domain and the second variable domain are paired in the antigen-binding region of the protein such that the antigen-binding region specifically binds folate receptor alpha (FRalpha).